It is wrapped in a user-friendly working environment that contains several settings to tinker with.
#Amplifx windows
MPprimer: a program for reliable multiplex PCR primer design.īMC Bioinformatics, 2010, 11:143 Posted on 1 4 Categories PCR / Primer Design Tags MPprimer, Multiplex, PCR, Primer Design Leave a comment on MPprimer 1.5 – Reliable Multiplex PCR Primer Design PCR Analyzer v 1.AmplifX is a Windows application designed to help you design new primers and browse primer collections, in order to amplify a fragment into a target sequence, for instance. Zhiyong Shen, Wubin Qu, Wen Wang, Yiming Lu, Yonghong Wu, Zhifeng Li, Xingyi Hang, Xiaolei Wang, Dongsheng Zhao, Chenggang Zhang. In short, MPprimer is a valuable tool for designing specific, no dimer formation and amplicons size constrained PSCs to improve the multiplex PCR experiments. In addition, MPprimer provides a virtual electrophotogram to help users choose the best PSC. The graph-expanding algorithm derived from the greedy algorithm was used to determine the optimal primer set combinations (PSCs) for multiplex PCR. MPprimer employs the widely used primer design program Primer3 and the primer specificity evaluation program MFEprimer to design and evaluate the candidate primers based on genomic or transcript DNA database, followed by careful examination to avoid primer dimerization. MPprimer: a program for reliable multiplex PCR primer design. (2004) 32 (1): 17-24 Posted on 0 0 Categories PCR / Primer Design Tags Amplification, Design Primer, GenoFrag, Long-range, PCR, Whole Genome Scanning Leave a comment on GenoFrag 2.1 – Design Primers Optimized for Whole Genome Scanning by Long-range PCR Amplification AmplifX 2.1.1 – Manage & Design Primers for PCR
#Amplifx software
GenoFrag: software to design primers optimized for whole genome scanning by long‐range PCR amplification This experimental validation demonstrates that GenoFrag is a robust and reliable tool for primer design and that whole genome PCR scanning can be envisaged for the analysis of genome diversity in S.aureus, one of the major public health concerns worldwide. Two subsets of primers were successfully used to amplify two portions of the N315 chromosome. A set of primers was generated from the genome sequence of S.aureus N315, employed here as a reference strain. GenoFrag was developed for the analysis of Staphylococcus aureus genome plasticity by whole genome amplification in ～10 kb‐long fragments. Posted on 4 4 Categories PCR / Primer Design Tags BatchPrimer3, High Throughput, PCR, Primer Design, Sequencing, Web Application RJPrimers 1.0 – Transposable Element Junction based PCR Primer Design You FM, Huo N, Gu YQ, Luo MC, Ma Y, Hane D, Lazo GR, Dvorak J, Anderson OD.īMC Bioinformatics. A batch input of large number of sequences and a tab-delimited result output greatly facilitates rapid primer design and ordering process.ĭepartment of Plant Sciences, University of CaliforniaīatchPrimer3: a high throughput web application for PCR and sequencing primer design. BatchPrimer3 allows users to design several types of primers including generic primers, hybridization oligos, SSR primers together with SSR detection, and SNP genotyping primers (including single-base extension primers, allele-specific primers, and tetra-primers for tetra-primer ARMS PCR), as well as DNA sequencing primers. BatchPrimer3 is a comprehensive web primer design program using Primer3 core program as a major primer design engine to design different types of PCR primers and sequencing primers in a high-through manner.
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